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Toolkit

MODS Testing for Tuberculosis

Online video protocol at the Journal of Visualized Experiments http://www.jove.com/index/Details.stp?ID=845 or download just the video here 52mb small or 169mb large in Quicktime format

*Nuevo: Vídeo del protocolo en español (subtitulos) pequeño o grande

The "MODS" (microscopic-observation drug-susceptibility) assay is a faster, cheaper, and more sensitive test than other culture-based tests currently in use for tuberculosis (TB) diagnosis. The philosophy underlying MODS is laboratory freeware - all the components are readily available from laboratory suppliers; there are no hidden, patented secrets. TB is primarily a disease of the poor which further impoverishes the most disadvantaged societies. This is a curable disease which, given the right tools correctly applied, we can control.

The discovery of the features of M tuberculosis growth detection which led to the development of MODS is attributed to Luz Caviedes working under the supervision of Bob Gilman in his laboratories at the Universidad Peruana Cayetano Heredia, Lima, Peru. The subsequent operational evaluation and refinement of the MODS methodology was the result of an international research collaboration involving the Universidad Peruana Cayetano Heredia (UPCH), Johns Hopkins Bloomberg School of Public Health and Imperial College London, based at UPCH and funded by The Wellcome Trust.

In partnership with the Peruvian National Reference Laboratory at the Instituto Nacional de Salud and the National TB Control Programme, this collaborative group is now leading the associated translational research, supported by the Wellcome Trust, which aims to answer the question “what is the optimal way to utilise MODS in a resource-limited programmatic setting?” Additional financial support for MODS implementation in Lima, Peru and Chennai, India is provided through a 2007 Development Marketplace award from the World Bank.

Here are PowerPoint* files of the progressive MTB growth patterns in MODS, as viewed through an inverted light microscope.

MTB in MODS x10 objective slide (776kb)

MTB in MODS x10 objective poster size (14.9mb)**

MTB in MODS x20 objective slide (390kb)

MTB in MODS x20 objective poster size (6.1mb)**

*PowerPoint 97-03 compatible

**The poster slides are very large (1.42 x1.07 meters) so that you can print them for your laboratory at whatever smaller size you choose

Please email modsperu@gmail.com for questions not addressed on this site or for technical assistance. We will reply to you as soon as possible. Please include:

1) your name and position

2) the location you are writing regarding

3) your questions or requests

El jurado agradece a todos los participantes por el envío de sus propuestas, así como por el trabajo y tiempo dedicado a la preparación de cada una. La calidad de los trabajos finales presentados impresionó a los jueces, y ha motivado una profunda discusión sobre las virtudes de cada uno de los diseños presentados. Sin embargo, ninguno de los diseños presentados logró abarcar todos los aspectos señalados en las bases del concurso. Las aéreas de control de temperatura, fuentes de energía, y necesidades de ventilación fueron las que causaron mayores problemas para todos los grupos. Pese a esto, se espera que el diseño final de laboratorio que se elaborará en los siguientes meses, incorpore algunos aspectos de cada uno de los diseños presentados.

Luego de un prolongado proceso de evaluación y discusión, el Jurado decidió que el diseño presentado por el Grupo conformado por Sergio Guzmán, Manuel Bauer e Iris Quintana es el ganador del "Concurso de Diseño de un Laboratorio Móvil de MODS". Este diseño es para el equipo de jueces el más factible y reproducible, además de incluir propuestas innovadoras.