Universidad Peruana Cayetano Heredia

Microscopic Observation Drug Susceptibility Assay

Antibiotic Working Solution Preparation

 

Flowchart: Alternate Process: Thaw aliquot* of INH storage stock and add 100l to 900l of 7H9/OADC
Flowchart: Alternate Process: Add 1ml of 40g/ml to 9ml of 7H9/OADC
(gives 10ml of 4g/ml)
Flowchart: Alternate Process: Plating 100l of 4 g/ml to 900l 7H9/OADC yields final concentration of 0.4g/ml
Flowchart: Alternate Process: Plating 100l of 10 g/ml to 900l 7H9/OADC yields final concentration of 1g/ml
Flowchart: Alternate Process: Add 1ml of 100g/ml to 9ml of 7H9/OADC
(gives 10ml of 10g/ml)
Flowchart: Alternate Process: Thaw aliquot* of RIF storage stock and add 100l to 900l of 7H9/OADC

STOCK No. 2

40g/ml

STOCK No. 2

100g/ml

Working Solution

*Once thawed, unused antibiotic must be discarded as properties may not be retained

Isoniazid

Rifampicin