Universidad Peruana Cayetano Heredia

Microscopic Observation Drug Susceptibility Assay

Middlebrook 7H9 Broth

with Casitone and Glycerol

Flowchart: Alternate Process:    3.1ml glycerol + 1.25g Bacto Casitone
Flowchart: Alternate Process: Resuspend 5.9g 7H9 media powder
Flowchart: Alternate Process: Autoclave at 121-124oC for 15 min
Flowchart: Alternate Process: Divide into 4.5ml aliquots, incubate at 37oC for 48 hours
Flowchart: Alternate Process: Store at 6-8oC for 
up to 1 month

Begin with 900ml deionized H2O

Lack of turbidity signifies a sterile solution

Flowchart: Alternate Process: Day of Use:
Add 0.5ml OADC