Universidad Peruana Cayetano Heredia

Microscopic Observation Drug Susceptibility Assay

Preparation of

Sputum Decontamination

Solution (200ml)

100ml sterile deionized H2O

100ml sterile deionized H2O

Flowchart: Alternate Process: 4.0g NaOH
Flowchart: Alternate Process: Day of Use:
Add 0.1g NALC crystals to every 20ml of decontamination solution
Flowchart: Alternate Process: Store at 6-8oC for 
up to 1 month
Flowchart: Alternate Process: Autoclave 121-124oC 
for 15 min
Flowchart: Alternate Process: 2.9g Sodium Citrate

Discard any unused solution after 24 hours