Universidad Peruana Cayetano Heredia

Microscopic Observation Drug Susceptibility Assay

Plating broth, sample, and antibiotics

Flowchart: Alternate Process: Resuspend pellet in 5ml final 7H9 medium
Flowchart: Alternate Process: Aliquot 900μl of broth/sample to 4 wells of a 24 well plate
Flowchart: Alternate Process: Add 0.1ml PANTA

Add 100μl INH

Add 100μl Rif

100μl 7H9 with OADC