Universidad Peruana Cayetano Heredia

Microscopic Observation Drug Susceptibility Assay

NaOH-NALC

Sputum Decontamination

Text Box: Begin with 2ml* 
of sputum sample
Flowchart: Alternate Process: Decant supernatant and retain pellet
Flowchart: Alternate Process: Centrifuge at 3000xg 
for 15 min
Flowchart: Alternate Process: Fill tube to 14ml with PBS. Cap tube and mix by inversion.	
Flowchart: Alternate Process: Vortex tube for 20 sec. and let stand for 15min.
Flowchart: Alternate Process: Add an equal amount of NaOH-NALC

* If 2ml is not available, add PBS up to 2ml; if more than 2ml is available, use only 2ml